• Enzyme-linked immunosorbent assays (ELISAs) are used extensively in biotechnology, pharmaceutical, and scientific drugs labs. On the identical time, they look like underrepresented in chemistry and biochemistry curricula, although their sensitivity, selectivity, and ease of use would argue for his or her widespread use.

• We describe right here an internet ELISA exercise appropriate for stand-alone use or along side an precise moist lab ELISA. Particularly, we provide actual and mock information for a hypothetical ELISA to detect plasma antibodies to COVID-19 in contaminated sufferers who’ve had the illness.

• A lot of the exercise focuses on chemical and mathematical fashions to suit ELISA or any macromolecule/ligand binding information, a talent that addresses maybe essentially the most related and tough studying objective of an ELISA experiment.

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

• Key phrases: distance studying; laboratory workouts; web-based studying.

Influenza A Virus H3N2 Brisbane 10 / 07 Protein
20-abx260509	Abbexa	EUR 276.00 EUR 3082.80 EUR 393.60	10 ug 1 mg 50 ug

Influenza A Strain A/Brisbane/10/07 (H3N2) Antigen
11-567	ProSci	0.1 mg	EUR 632.4
Description: Influenza A (A/Brisbane/10/07), Influenza A Virus, Strain A/Brisbane/10/07 (H3N2)

Histone H3 Polyclonal Antibody
A-1112	EpiGentek	EUR 702.06 EUR 435.60	100 µg 100 ul

Phospho-Histone H3 (Ser28) Polyclonal Antibody
A-4050	EpiGentek	EUR 684.66 EUR 302.50 EUR 423.50	100 µg 50 ul 100 ul

Rabbit Anti-Influenza A HA (H3N2, 1-531aa/HA1+HA2) (A/Brisbane/10/2007) protein IgG, aff pure
H3N23-A	Alpha Diagnostics	100 ul	EUR 534

Phospho-Histone H3 (Thr3) Polyclonal Antibody
A-4051	EpiGentek	EUR 684.66 EUR 423.50	100 µg 100 ul

Phospho-Histone H3 (Thr11) Polyclonal Antibody
A-4052	EpiGentek	EUR 684.66 EUR 423.50	100 µg 100 ul

Influenza A H3 Antibody
abx021977-1mg	Abbexa	1 mg	EUR 1053.6

Influenza A H3 Antibody
abx021978-1mg	Abbexa	1 mg	EUR 1053.6

Influenza A antibody
10-1096	Fitzgerald	500 ug	EUR 249.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1097	Fitzgerald	1 mg	EUR 418.8
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1098	Fitzgerald	1 mg	EUR 726
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1099	Fitzgerald	1 mg	EUR 726
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1100	Fitzgerald	500 ug	EUR 418.8
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1101	Fitzgerald	1 mg	EUR 726
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1102	Fitzgerald	500 ug	EUR 418.8
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1103	Fitzgerald	500 ug	EUR 418.8
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1241	Fitzgerald	500 ug	EUR 418.8
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1243	Fitzgerald	500 ug	EUR 418.8
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-7866	Fitzgerald	1 mg	EUR 483.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-7867	Fitzgerald	1 mg	EUR 483.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50A	Fitzgerald	1 mg	EUR 603.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50B	Fitzgerald	1 mg	EUR 470.4
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50C	Fitzgerald	1 mg	EUR 470.4
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50D	Fitzgerald	1 mg	EUR 334.8
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50E	Fitzgerald	1 mg	EUR 334.8
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50F	Fitzgerald	1 mg	EUR 327.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50G	Fitzgerald	1 mg	EUR 489.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50H	Fitzgerald	1 mg	EUR 649.2
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50I	Fitzgerald	1 mg	EUR 489.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50K	Fitzgerald	1 mg	EUR 327.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50L	Fitzgerald	1 mg	EUR 360
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50M	Fitzgerald	1 mg	EUR 360
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50N	Fitzgerald	1 mg	EUR 360
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50O	Fitzgerald	1 mg	EUR 360
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50P	Fitzgerald	1 mg	EUR 360
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50Q	Fitzgerald	1 mg	EUR 327.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50R	Fitzgerald	1 mg	EUR 327.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50S	Fitzgerald	1 mg	EUR 327.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50T	Fitzgerald	1 mg	EUR 327.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50U	Fitzgerald	1 mg	EUR 327.6
Description: Mouse monoclonal Influenza A antibody

Influenza A Hemagglutinin H1 (A/Brisbane/59/2007)(H1N1) Peptide
11-563	ProSci	0.05 mg	EUR 632.4
Description: Influenza A Hemagglutinin H1 (A/Brisbane/59/2007)(H1N1) Peptide

Influenza A Hemagglutinin H1 (A/Brisbane/59/2007)(H1N1) Peptide
11-564	ProSci	0.05 mg	EUR 632.4
Description: Influenza A Hemagglutinin H1 (A/Brisbane/59/2007)(H1N1) Peptide

Lung Tissue Lysate (Tumor)
1703-07	ProSci	0.1 mg	EUR 336.3
Description: Lung tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Tumor)
1707-07	ProSci	0.1 mg	EUR 336.3
Description: Kidney tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Breast Tissue Lysate (Normal)
1710-07	ProSci	0.1 mg	EUR 260.7
Description: Breast tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Breast Tissue Lysate (Tumor)
1714-07	ProSci	0.1 mg	EUR 336.3
Description: Breast tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)
1715-07	ProSci	0.1 mg	EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Influenza A Virus H3 Antigen Antibody
abx415738-01mg	Abbexa	0.1 mg	EUR 861.6

Influenza A virus H3 PCR kit
PCR-H649-48R	Bioingentech	50T	EUR 987.6

Influenza A virus H3 PCR kit
PCR-H649-96R	Bioingentech	100T	EUR 1335.6

Influenza A virus fragment
A1075-10	ApexBio	10 mg	EUR 338.4
Description: Influenza A virus fragment has the RNA sequence of Leu-Lys-Phe-Ala-Phe-Ser-Met-Met.Influenzavirus A is agenusof the Orthomyxoviridaefamily of viruses.

IAV H3N2 nucleoprotein (A/Brisbane/10/2007) [His]
DAG2329	Creative Diagnostics	100 µg	EUR 868.8

Mouse Monoclonal Anti-Influenza A HA (H3N2, 1-531aa/HA1+HA2) (A/Brisbane/10/2007) protein IgG
H3N21-M	Alpha Diagnostics	100 ul	EUR 578.4

Influenza A HA H1 antibody
10-7839	Fitzgerald	500 ug	EUR 349.2
Description: Mouse monoclonal Influenza A haemagglutinin H1 antibody

Influenza A HA H1 antibody
10-7840	Fitzgerald	500 ug	EUR 349.2
Description: Mouse monoclonal Influenza A haemagglutinin H1 antibody

Influenza A HA H5 antibody
10-7854	Fitzgerald	1 mg	EUR 878.4
Description: Mouse monoclonal Influenza A HA H5 antibody

Influenza A HA H5 antibody
10-7855	Fitzgerald	1 mg	EUR 878.4
Description: Mouse monoclonal Influenza A HA H5 antibody

Influenza A HA H5 antibody
10-7856	Fitzgerald	1 mg	EUR 878.4
Description: Mouse monoclonal Influenza A HA H5 antibody

Influenza A virus H3 RT PCR kit
RTq-H649-100R	Bioingentech	100T	EUR 1573.2

Influenza A virus H3 RT PCR kit
RTq-H649-150R	Bioingentech	150T	EUR 2144.4

Influenza A virus H3 RT PCR kit
RTq-H649-50R	Bioingentech	50T	EUR 1155.6

Influenza B Virus Florida 07/04
7-07918	CHI Scientific	10µg	Ask for price

Influenza B Virus Florida 07/04
7-07919	CHI Scientific	50µg	Ask for price

Influenza B Virus Florida 07/04
7-07920	CHI Scientific	1mg	Ask for price

hemagglutinin (332-340) [Influenza A virus]
A1141-10	ApexBio	10 mg	EUR 408
Description: Influenza hemagglutinin (HA) is a type of hemagglutinin found on the surface of the influenza viruses. It is an antigenic glycoprotein. It is responsible for binding the virus to the cell that is being infected.

Influenza A virus H3 One-Step PCR kit
Oneq-H649-100R	Bioingentech	100T	EUR 1932

Influenza A virus H3 One-Step PCR kit
Oneq-H649-150R	Bioingentech	150T	EUR 2646

• Background: COVID-19 pandemic has unfold worldwide since December 2019. Serological assessments for SARS-CoV-2 antibody testing are wanted for detection of present or previous infections. A variety of business assessments is accessible. Nonetheless, most of them have to be validated.

• Research design: The goal was to match a business IgG and IgA ELISA (Euroimmun) with three lateral stream immunoassays (LFI): Hangzhou Alltest Biotech, Wuhan UNscience Biotechnology and Guangzhou Wondfo Biotech. Specificity was calculated with 62 out there serum samples from 2018/19. The examine included 152 sera from sufferers of which 109 have been RT-PCR constructive. Sensitivities for ELISA anti SARS-CoV-2 IgG and IgA have been 81.5 % and 93.1 % and specificities 100 % and 80.6 %, respectively. LFI confirmed variable performances, general outcomes being higher for Guangzhou Wondfo Biotech.

• Conclusions: Industrial serological assessments are helpful for detection of antibodies in sufferers with COVID-19. ELISA introduced higher outcomes than LFI. The outcomes allowed to include essentially the most delicate LFI to the day by day workflow, combining with ELISA. Cautious validation is inspired earlier than scientific laboratories begin utilizing these assessments.

• Key phrases: Antibody detection; COVID-19; Analysis; SARS-CoV-2; Sensitivity; Specificity.