• Enzyme-linked immunosorbent assays (ELISAs) are used extensively in biotechnology, pharmaceutical, and scientific drugs labs. On the identical time, they look like underrepresented in chemistry and biochemistry curricula, although their sensitivity, selectivity, and ease of use would argue for his or her widespread use.

• We describe right here an internet ELISA exercise appropriate for stand-alone use or along side an precise moist lab ELISA. Particularly, we provide actual and mock information for a hypothetical ELISA to detect plasma antibodies to COVID-19 in contaminated sufferers who’ve had the illness.

• A lot of the exercise focuses on chemical and mathematical fashions to suit ELISA or any macromolecule/ligand binding information, a talent that addresses maybe essentially the most related and tough studying objective of an ELISA experiment.

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

• Key phrases: distance studying; laboratory workouts; web-based studying.

Influenza A Virus H3N2 Brisbane 10 / 07 Protein
20-abx260509	Abbexa	EUR 230.00 EUR 2569.00 EUR 328.00	10 ug 1 mg 50 ug

Influenza A Strain A/Brisbane/10/07 (H3N2) Antigen
11-567	ProSci	0.1 mg	EUR 527
Description: Influenza A (A/Brisbane/10/07), Influenza A Virus, Strain A/Brisbane/10/07 (H3N2)

Rabbit Anti-Influenza A HA (H3N2, 1-531aa/HA1+HA2) (A/Brisbane/10/2007) protein IgG, aff pure
H3N23-A	Alpha Diagnostics	100 ul	EUR 445

Influenza A H3 Antibody
abx021977-1mg	Abbexa	1 mg	EUR 878

Influenza A H3 Antibody
abx021978-1mg	Abbexa	1 mg	EUR 878

Lung Tissue Lysate (Tumor)
1703-07	ProSci	0.1 mg	EUR 280.25
Description: Lung tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Tumor)
1707-07	ProSci	0.1 mg	EUR 280.25
Description: Kidney tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Breast Tissue Lysate (Normal)
1710-07	ProSci	0.1 mg	EUR 217.25
Description: Breast tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Breast Tissue Lysate (Tumor)
1714-07	ProSci	0.1 mg	EUR 280.25
Description: Breast tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)
1715-07	ProSci	0.1 mg	EUR 217.25
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Influenza A Hemagglutinin H1 (A/Brisbane/59/2007)(H1N1) Peptide
11-563	ProSci	0.05 mg	EUR 527
Description: Influenza A Hemagglutinin H1 (A/Brisbane/59/2007)(H1N1) Peptide

Influenza A Hemagglutinin H1 (A/Brisbane/59/2007)(H1N1) Peptide
11-564	ProSci	0.05 mg	EUR 527
Description: Influenza A Hemagglutinin H1 (A/Brisbane/59/2007)(H1N1) Peptide

Influenza A antibody
10-1096	Fitzgerald	500 ug	EUR 208
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1097	Fitzgerald	1 mg	EUR 349
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1098	Fitzgerald	1 mg	EUR 605
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1099	Fitzgerald	1 mg	EUR 605
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1100	Fitzgerald	500 ug	EUR 349
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1101	Fitzgerald	1 mg	EUR 605
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1102	Fitzgerald	500 ug	EUR 349
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1103	Fitzgerald	500 ug	EUR 349
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1241	Fitzgerald	500 ug	EUR 349
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1243	Fitzgerald	500 ug	EUR 349
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-7866	Fitzgerald	1 mg	EUR 403
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-7867	Fitzgerald	1 mg	EUR 403
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50A	Fitzgerald	1 mg	EUR 503
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50B	Fitzgerald	1 mg	EUR 392
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50C	Fitzgerald	1 mg	EUR 392
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50D	Fitzgerald	1 mg	EUR 279
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50E	Fitzgerald	1 mg	EUR 279
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50F	Fitzgerald	1 mg	EUR 273
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50G	Fitzgerald	1 mg	EUR 408
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50H	Fitzgerald	1 mg	EUR 541
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50I	Fitzgerald	1 mg	EUR 408
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50K	Fitzgerald	1 mg	EUR 273
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50L	Fitzgerald	1 mg	EUR 300
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50M	Fitzgerald	1 mg	EUR 300
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50N	Fitzgerald	1 mg	EUR 300
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50O	Fitzgerald	1 mg	EUR 300
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50P	Fitzgerald	1 mg	EUR 300
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50Q	Fitzgerald	1 mg	EUR 273
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50R	Fitzgerald	1 mg	EUR 273
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50S	Fitzgerald	1 mg	EUR 273
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50T	Fitzgerald	1 mg	EUR 273
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50U	Fitzgerald	1 mg	EUR 273
Description: Mouse monoclonal Influenza A antibody

Influenza A virus H3 PCR kit
PCR-H649-48R	Bioingentech	50T	EUR 823

Influenza A virus H3 PCR kit
PCR-H649-96R	Bioingentech	100T	EUR 1113

Influenza A Virus H3 Antigen Antibody
abx415738-01mg	Abbexa	0.1 mg	EUR 718

Influenza A virus fragment
A1075-10	ApexBio	10 mg	EUR 282
Description: Influenza A virus fragment has the RNA sequence of Leu-Lys-Phe-Ala-Phe-Ser-Met-Met.Influenzavirus A is agenusof the Orthomyxoviridaefamily of viruses.

IAV H3N2 nucleoprotein (A/Brisbane/10/2007) [His]
DAG2329	Creative Diagnostics	100 µg	EUR 724

Influenza B Virus Florida 07/04
7-07918	CHI Scientific	10µg	Ask for price

Influenza B Virus Florida 07/04
7-07919	CHI Scientific	50µg	Ask for price

Influenza B Virus Florida 07/04
7-07920	CHI Scientific	1mg	Ask for price

Mouse Monoclonal Anti-Influenza A HA (H3N2, 1-531aa/HA1+HA2) (A/Brisbane/10/2007) protein IgG
H3N21-M	Alpha Diagnostics	100 ul	EUR 482

Influenza A virus H3 RT PCR kit
RTq-H649-100R	Bioingentech	100T	EUR 1311

Influenza A virus H3 RT PCR kit
RTq-H649-150R	Bioingentech	150T	EUR 1787

Influenza A virus H3 RT PCR kit
RTq-H649-50R	Bioingentech	50T	EUR 963

Influenza A HA H1 antibody
10-7839	Fitzgerald	500 ug	EUR 291
Description: Mouse monoclonal Influenza A haemagglutinin H1 antibody

Influenza A HA H1 antibody
10-7840	Fitzgerald	500 ug	EUR 291
Description: Mouse monoclonal Influenza A haemagglutinin H1 antibody

Influenza A HA H5 antibody
10-7854	Fitzgerald	1 mg	EUR 732
Description: Mouse monoclonal Influenza A HA H5 antibody

Influenza A HA H5 antibody
10-7855	Fitzgerald	1 mg	EUR 732
Description: Mouse monoclonal Influenza A HA H5 antibody

Influenza A HA H5 antibody
10-7856	Fitzgerald	1 mg	EUR 732
Description: Mouse monoclonal Influenza A HA H5 antibody

Influenza B (B / Florida / 07 / 04) Protein
abx060630-1mg	Abbexa	1 mg	EUR 1873

Influenza B Virus Florida 07 / 04 Protein
20-abx260511	Abbexa	EUR 230.00 EUR 2569.00 EUR 328.00	10 ug 1 mg 50 ug

Influenza A virus H3 One-Step PCR kit
Oneq-H649-100R	Bioingentech	100T	EUR 1610

Influenza A virus H3 One-Step PCR kit
Oneq-H649-150R	Bioingentech	150T	EUR 2205

Influenza A virus H3 One-Step PCR kit
Oneq-H649-50R	Bioingentech	50T	EUR 1175

IAV H3N2 H3 (aa 17-529) (A/Panama/07/99) [His]
DAG2162	Creative Diagnostics	100 µg	Ask for price

hemagglutinin (332-340) [Influenza A virus]
A1141-10	ApexBio	10 mg	EUR 340
Description: Influenza hemagglutinin (HA) is a type of hemagglutinin found on the surface of the influenza viruses. It is an antigenic glycoprotein. It is responsible for binding the virus to the cell that is being infected.

• Background: COVID-19 pandemic has unfold worldwide since December 2019. Serological assessments for SARS-CoV-2 antibody testing are wanted for detection of present or previous infections. A variety of business assessments is accessible. Nonetheless, most of them have to be validated.

• Research design: The goal was to match a business IgG and IgA ELISA (Euroimmun) with three lateral stream immunoassays (LFI): Hangzhou Alltest Biotech, Wuhan UNscience Biotechnology and Guangzhou Wondfo Biotech. Specificity was calculated with 62 out there serum samples from 2018/19. The examine included 152 sera from sufferers of which 109 have been RT-PCR constructive. Sensitivities for ELISA anti SARS-CoV-2 IgG and IgA have been 81.5 % and 93.1 % and specificities 100 % and 80.6 %, respectively. LFI confirmed variable performances, general outcomes being higher for Guangzhou Wondfo Biotech.

• Conclusions: Industrial serological assessments are helpful for detection of antibodies in sufferers with COVID-19. ELISA introduced higher outcomes than LFI. The outcomes allowed to include essentially the most delicate LFI to the day by day workflow, combining with ELISA. Cautious validation is inspired earlier than scientific laboratories begin utilizing these assessments.

• Key phrases: Antibody detection; COVID-19; Analysis; SARS-CoV-2; Sensitivity; Specificity.