• Enzyme-linked immunosorbent assays (ELISAs) are used extensively in biotechnology, pharmaceutical, and scientific drugs labs. On the identical time, they look like underrepresented in chemistry and biochemistry curricula, although their sensitivity, selectivity, and ease of use would argue for his or her widespread use.

• We describe right here an internet ELISA exercise appropriate for stand-alone use or along side an precise moist lab ELISA. Particularly, we provide actual and mock information for a hypothetical ELISA to detect plasma antibodies to COVID-19 in contaminated sufferers who’ve had the illness.

• A lot of the exercise focuses on chemical and mathematical fashions to suit ELISA or any macromolecule/ligand binding information, a talent that addresses maybe essentially the most related and tough studying objective of an ELISA experiment.

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

• Key phrases: distance studying; laboratory workouts; web-based studying.

Influenza A Virus H3N2 Brisbane 10 / 07 Protein
20-abx260509	Abbexa	EUR 276.00 EUR 3082.80 EUR 393.60	10 ug 1 mg 50 ug

Influenza A Strain A/Brisbane/10/07 (H3N2) Antigen
11-567	ProSci	0.1 mg	EUR 632.4
Description: Influenza A (A/Brisbane/10/07), Influenza A Virus, Strain A/Brisbane/10/07 (H3N2)

Histone H3 Polyclonal Antibody
A-1112	EpiGentek	EUR 702.06 EUR 435.60	100 µg 100 ul

Phospho-Histone H3 (Ser28) Polyclonal Antibody
A-4050	EpiGentek	EUR 684.66 EUR 302.50 EUR 423.50	100 µg 50 ul 100 ul

Phospho-Histone H3 (Thr3) Polyclonal Antibody
A-4051	EpiGentek	EUR 684.66 EUR 423.50	100 µg 100 ul

Phospho-Histone H3 (Thr11) Polyclonal Antibody
A-4052	EpiGentek	EUR 684.66 EUR 423.50	100 µg 100 ul

Rabbit Anti-Influenza A HA (H3N2, 1-531aa/HA1+HA2) (A/Brisbane/10/2007) protein IgG, aff pure
H3N23-A	Alpha Diagnostics	100 ul	EUR 534

Influenza A H3 Antibody
abx021977-1mg	Abbexa	1 mg	EUR 1053.6

Influenza A H3 Antibody
abx021978-1mg	Abbexa	1 mg	EUR 1053.6

Lung Tissue Lysate (Tumor)
1703-07	ProSci	0.1 mg	EUR 336.3
Description: Lung tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Tumor)
1707-07	ProSci	0.1 mg	EUR 336.3
Description: Kidney tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Breast Tissue Lysate (Normal)
1710-07	ProSci	0.1 mg	EUR 260.7
Description: Breast tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Breast Tissue Lysate (Tumor)
1714-07	ProSci	0.1 mg	EUR 336.3
Description: Breast tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)
1715-07	ProSci	0.1 mg	EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Influenza A antibody
10-1096	Fitzgerald	500 ug	EUR 249.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1097	Fitzgerald	1 mg	EUR 418.8
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1098	Fitzgerald	1 mg	EUR 726
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1099	Fitzgerald	1 mg	EUR 726
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1100	Fitzgerald	500 ug	EUR 418.8
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1101	Fitzgerald	1 mg	EUR 726
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1102	Fitzgerald	500 ug	EUR 418.8
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1103	Fitzgerald	500 ug	EUR 418.8
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1241	Fitzgerald	500 ug	EUR 418.8
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-1243	Fitzgerald	500 ug	EUR 418.8
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-7866	Fitzgerald	1 mg	EUR 483.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-7867	Fitzgerald	1 mg	EUR 483.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50A	Fitzgerald	1 mg	EUR 603.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50B	Fitzgerald	1 mg	EUR 470.4
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50C	Fitzgerald	1 mg	EUR 470.4
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50D	Fitzgerald	1 mg	EUR 334.8
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50E	Fitzgerald	1 mg	EUR 334.8
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50F	Fitzgerald	1 mg	EUR 327.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50G	Fitzgerald	1 mg	EUR 489.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50H	Fitzgerald	1 mg	EUR 649.2
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50I	Fitzgerald	1 mg	EUR 489.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50K	Fitzgerald	1 mg	EUR 327.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50L	Fitzgerald	1 mg	EUR 360
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50M	Fitzgerald	1 mg	EUR 360
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50N	Fitzgerald	1 mg	EUR 360
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50O	Fitzgerald	1 mg	EUR 360
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50P	Fitzgerald	1 mg	EUR 360
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50Q	Fitzgerald	1 mg	EUR 327.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50R	Fitzgerald	1 mg	EUR 327.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50S	Fitzgerald	1 mg	EUR 327.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50T	Fitzgerald	1 mg	EUR 327.6
Description: Mouse monoclonal Influenza A antibody

Influenza A antibody
10-I50U	Fitzgerald	1 mg	EUR 327.6
Description: Mouse monoclonal Influenza A antibody

Influenza A Hemagglutinin H1 (A/Brisbane/59/2007)(H1N1) Peptide
11-563	ProSci	0.05 mg	EUR 632.4
Description: Influenza A Hemagglutinin H1 (A/Brisbane/59/2007)(H1N1) Peptide

Influenza A Hemagglutinin H1 (A/Brisbane/59/2007)(H1N1) Peptide
11-564	ProSci	0.05 mg	EUR 632.4
Description: Influenza A Hemagglutinin H1 (A/Brisbane/59/2007)(H1N1) Peptide

MBD1 Polyclonal Antibody
A-1006	EpiGentek	EUR 740.34 EUR 117.70 EUR 306.90 EUR 462.00	100 µg 20 ul 50 ul 100 ul

5-Methylcytosine (5-mC) Monoclonal Antibody [33D3]
A-1014	EpiGentek	EUR 721.20 EUR 82.50 EUR 239.80 EUR 456.50	100 µg 10 ug 50 ug 100 ug

5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody [HMC/4D9]
A-1018	EpiGentek	EUR 702.06 EUR 82.50 EUR 239.80 EUR 443.30	100 µg 10 ug 50 ug 100 ug

PRDM4 Polyclonal Antibody
A-2004	EpiGentek	EUR 754.26 EUR 117.70 EUR 284.90 EUR 470.80	100 µg 20 ul 50 ul 100 ul

PRDM5 Polyclonal Antibody
A-2005	EpiGentek	EUR 754.26 EUR 117.70 EUR 284.90 EUR 470.80	100 µg 20 ul 50 ul 100 ul

PRDM10 Polyclonal Antibody
A-2010	EpiGentek	EUR 754.26 EUR 117.70 EUR 284.90 EUR 470.80	100 µg 20 ul 50 ul 100 ul

PRDM11 Polyclonal Antibody
A-2011	EpiGentek	EUR 754.26 Ask for price Ask for price EUR 470.80	100 µg 20 ul 50 ul 100 ul

PRDM13 Polyclonal Antibody
A-2013	EpiGentek	EUR 754.26 Ask for price Ask for price EUR 470.80	100 µg 20 ul 50 ul 100 ul

PRDM16 Polyclonal Antibody
A-2016	EpiGentek	EUR 754.26 EUR 117.70 EUR 284.90 EUR 470.80	100 µg 20 ul 50 ul 100 ul

PRDM17 Polyclonal Antibody
A-2017	EpiGentek	EUR 754.26 EUR 117.70 EUR 284.90 EUR 470.80	100 µg 20 ul 50 ul 100 ul

EZH1 Polyclonal Antibody
A-2018	EpiGentek	EUR 754.26 EUR 117.70 EUR 284.90 EUR 470.80	100 µg 20 ul 50 ul 100 ul

HSF1 Polyclonal Antibody
A-2401	EpiGentek	EUR 905.64 EUR 235.40 EUR 423.50 EUR 574.20	200 µl 50 ul 100 ul 200 ul

PRMT3 Polyclonal Antibody
A-3003	EpiGentek	EUR 740.34 Ask for price Ask for price EUR 462.00	100 µg 20 ul 50 ul 100 ul

SET1 Polyclonal Antibody
A-3011	EpiGentek	EUR 754.26 EUR 117.70 EUR 284.90 EUR 470.80	100 µg 20 ul 50 ul 100 ul

SET07 Polyclonal Antibody
A-3013	EpiGentek	EUR 754.26 Ask for price Ask for price EUR 470.80	100 µg 20 ul 50 ul 100 ul

LSD1 Polyclonal Antibody
A-3018	EpiGentek	EUR 754.26 EUR 117.70 EUR 284.90 EUR 470.80	100 µg 20 ul 50 ul 100 ul

HDAC10 Polyclonal Antibody
A-4010	EpiGentek	EUR 754.26 Ask for price Ask for price EUR 470.80	100 µg 20 ul 50 ul 100 ul

PCAF Polyclonal Antibody
A-4012	EpiGentek	EUR 740.34 EUR 117.70 EUR 306.90 EUR 462.00	100 µg 20 ul 50 ul 100 ul

Histone H3K9ac (Acetyl H3K9) Polyclonal Antibody
A-4022	EpiGentek	EUR 555.90 EUR 173.80 EUR 336.60 EUR 470.80	50 µl 25 ul 50 ul 100 ul

Histone H3K14ac (Acetyl H3K14) Polyclonal Antibody
A-4023	EpiGentek	EUR 555.90 EUR 173.80 EUR 336.60 EUR 470.80	50 µl 25 ul 50 ul 100 ul

• Background: COVID-19 pandemic has unfold worldwide since December 2019. Serological assessments for SARS-CoV-2 antibody testing are wanted for detection of present or previous infections. A variety of business assessments is accessible. Nonetheless, most of them have to be validated.

• Research design: The goal was to match a business IgG and IgA ELISA (Euroimmun) with three lateral stream immunoassays (LFI): Hangzhou Alltest Biotech, Wuhan UNscience Biotechnology and Guangzhou Wondfo Biotech. Specificity was calculated with 62 out there serum samples from 2018/19. The examine included 152 sera from sufferers of which 109 have been RT-PCR constructive. Sensitivities for ELISA anti SARS-CoV-2 IgG and IgA have been 81.5 % and 93.1 % and specificities 100 % and 80.6 %, respectively. LFI confirmed variable performances, general outcomes being higher for Guangzhou Wondfo Biotech.

• Conclusions: Industrial serological assessments are helpful for detection of antibodies in sufferers with COVID-19. ELISA introduced higher outcomes than LFI. The outcomes allowed to include essentially the most delicate LFI to the day by day workflow, combining with ELISA. Cautious validation is inspired earlier than scientific laboratories begin utilizing these assessments.

• Key phrases: Antibody detection; COVID-19; Analysis; SARS-CoV-2; Sensitivity; Specificity.